Search results for "Mitotic chromosome"
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Chromosome numbers in Hieracium and Pilosella species (Asteraceae) from the Iberian Peninsula and the Balearic Islands
2007
Mitotic chromosome numbers are reported from 16 Hieracium and two Pilosella species from the Iberian Peninsula and the Balearic Islands that are little known cytogenetically. The chromosome numbers of Hieracium aragonense Scheele (2 n = 27, 36), H. compositum Lapeyr. (2 n = 27), H. murcandidum G. Mateo (2 n = 27), H. spathulatum Scheele (2 n = 27), H. segurae Mateo (2 n = 27), H. teruelanum Mateo (2 n = 27), H. valentinum Pau (2 n = 27), Pilosella pseudovahlii (De Retz) Mateo (2 n = 18), and P. tardans (Peter) Sojak (2 n = 36) were determined for the first time. New cytotypes were detected in H. cordifolium Lapeyr. (2 n = 27) and H. loscosianum Scheele (2 n = 36). The karyo- type of the stu…
Flow Cytometry and Karyotype Analysis ofD. melanogasterEye Disc Cells
2008
The developing Drosophila eye-antennal disc is a particularly suited system for the genetic and cellular studies of complex biological processes. Methods to analyze Drosophila eye discs by flow cytometry are mainly based on the dissociation of tissues with trypsin. Dissociation operated by trypsin is very effective, though it causes a lot of stress to live cells often compromising the use of treated cells for further analyses. Here, we report a method to produce dissociated eye-disc cells that retain cell-membrane markers and that can be used for flow cytometry and cytological analysis of mitotic chromosomes. The method described is a great complementing tool for the cellular characterizati…